Rapid Testing for Vibrio vulnificus & V. parahaemolyticus

Secure Food Solutions has patent-pending microbiological tests to detect and enumerate Vibrio vulnificus and Vibrio parahaemolyticus in shellfish and environmental samplesOur Vibrio test method is fast, reliable, low cost and easy to use.

Availability of such methods will expand the number and types of laboratories able to test for V. vulnificus and V. parahaemolyticus.  The tests will make Vibrio testing more accessible to states for reopening sampling, as well as oyster processors and post harvest processors. Adoption of these methods will also allow more states to generate background data on Vibrio levels for water quality monitoring and risk evaluations.   

Each test is uniquely formulated with enrichment broth on top of agar layers set in a multi-well plate format for rapid detection (color change) and enumeration (standard serial dilutions and MPN method). Test benefits include:

  • Accuracy:  High sensitivity and specificity.

  • 24-hour Results:  No pre-enrichment required.

  • Low Cost:  No expensive equipment required.

  • Easy to Use: Technician friendly, even for non-specialized personnel; All testing done in single plate for smaller footprint (usage and disposal) than current culture methods.

96-well plate, Vibrio vulnificus

Positive shows black; Negative shows yellow/brown

Positive shows black; Negative shows yellow/brown

96-well plate, Vibrio parahaemolyticus (pathogenic)

Positive shows pink; Negative shows yellow

Positive shows pink; Negative shows yellow

96-well plate, Vibrio parahaemolyticus (total)

Positive shows yellow; Negative shows blue/green

Positive shows yellow; Negative shows blue/green